Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Hall
Date created: 2008-10-06 11:00:00
Date modified: 2015-05-08 01:09:37
PcstA+rbs+xylE
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_K118021_sequence (Version 1) |
Description
xylE is from the Pseudomonas putida TOL (naphthalene and xylene degradadative plasmid) pWW0. This gene encodes the enzyme catechol-2,3-dioxygenase (metapyrocatechase), which converts catechol to the bright yellow product 2-hydroxy-cis,cis-muconic semialdehyde. This is a useful reporter gene; colonies or broths expressing active XylE, in the presence of oxygen, will rapidly convert catechol, a cheap colourless substrate, to a bright yellow compound with an absorbance maximum around 377 nm. The part includes the native ribosome binding site, so simply has been added to the glucose-repressible promoter of cstA. This allows for characterisation of pCstA over a range of glucose concentrations.Notes
No special considerationsSource
The template DNA for xylE was kindly supplied by Dr. Peter Williams of the University of Wales, Bangor.pCstA is from Escherichia coli JM109 genomic DNA.
| Access | Instance | Definition |
| public public | BBa_K118011 BBa_J33204 | BBa_K118011 BBa_J33204 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K118011 BBa_J33204 | 1,131 140,1097 | BBa_K118011 BBa_J33204 |