Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alişan Kayab??len
Date created: 2013-09-19 11:00:00
Date modified: 2015-06-08 11:15:36
Anti MazF construct (pHyperspank + Anti MazF + Double Terminator)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1197001_sequence (Version 1) |
Description
Anti MazF is an antisense RNA producing sequence which is complemantary to MazF (K302033). MazF is a non-specific ribonuclease coding element which kills Bacillus subtilis. Anti MazF element produces an mRNA which is reverse complemantary to mRNA produced by MazF. Thus, Anti Mazf mRNA binds to MazF mRNA and inhibit its activity for coding MazF protein.With pHyperspank promoter, the production of Anti MazF will be depend on IPTG presence in the medium. If there is IPTG in medium, it will inactivate LacI which inhibit pHyperspank promoter. Thus, Anti MazF will be produced. On the other hand, if there is no IPTG in medium, LacI will inhibit pHyperspank and Anti MazF mRNA cannot be produced. As a result MazF protein will be produced, and kills the bacteria.
Notes
Anti MazF is designed as a complemantary sequence to the last 4 bases of the RBS sequence, 6 bases to the intermediate region and to the first 40 bases of MazF coding sequence, tottally 50 bases.Source
It is synthesized as a reverse complementary sequence to MazF (K302033)| Access | Instance | Definition |
| public public public | BBa_K606040 BBa_K1197000 BBa_B0015 | pHS BBa_K1197000 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K606040 BBa_K1197000 BBa_B0015 | 1,92 101,150 159,287 | pHS BBa_K1197000 BBa_B0015 |