Types | DnaRegion
|
Roles | Composite
engineered_region
|
Sequences | BBa_K1212015_sequence (Version 1)
|
Description
TAL repressor expression under the control of a pBAD promoter and a theophylline riboswitch inducible by arabinose and theophylline.
Notes
There cannot be a scar between the end of the riboswitch and the start codon of the TAL repressor.
Source
The pBAD is a part from the registry. The riboswitch is from the Gallivan Lab's paper Synthetic riboswitches that induce gene expression in diverse bacterial species. by Topp et al. in Appl Environ Microbiol. The TAL repressor is from the Segal Lab at the UC Davis Genome Center. See their paper Quantitative Analysis of TALE-DNA Interactions Suggests Polarity Effects. by Meckler et al. submitted to Nucleic Acids Res.