BBa_K1212016

BBa_K1212016 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1212016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Amy Soon

Date created: 2013-09-15 11:00:00

Date modified: 2015-05-08 01:09:41

pTet + TBS2 + RBS + GFP + T

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1212016_sequence (Version 1)

Description

pTet promoter upstream of TALe binding site 2 (corresponding to TAL 8 (BBa_K1212007), with B0034 and Golden Gate compatible GFP.

Notes

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Source

The pTet promoter, RBS and GFP are all parts from the registry. The TALe binding site is from the Segal Lab at the UC Davis Genome Center. See their paper Quantitative Analysis of TALE-DNA Interactions Suggests Polarity Effects. by Meckler et al. submitted to Nucleic Acids Res.

Components

Access	Instance	Definition
public	BBa_R0040	p(tetR)
public	BBa_K1212005	BBa_K1212005
public	BBa_B0034	BBa_B0034
public	BBa_K1212008	BBa_K1212008
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	p(tetR)
BBa_K1212005	63,81	BBa_K1212005
BBa_B0034	90,101	BBa_B0034
BBa_K1212008	108,866	BBa_K1212008
BBa_B0015	875,1003	BBa_B0015

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1212016/1

Member of these Collections

• iGEM Parts Registry