BBa_K1217000

BBa_K1217000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1217000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ma Tsz Shan
Date created: 2013-09-14 11:00:00
Date modified: 2015-05-08 01:09:42

pT7-ppk1(T.forsythia)



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1217000_sequence (Version 1)

Description

Polyphosphate kinase (ppk1) (E.C. 2.7.4.1) catalyzes the formation of polyphosphate from ATP. In BBa_K1217000, ppk1 was under the control of T7 promotor. Under iptg induction, over-expression of ppk1 can reduce the phosphate level in the medium by its incorporation into polyphosphate synthesis inside the bacteria.

Notes

We choose to amplify ppk1 gene from Tannerella forsythia (ATCC 43037) since the sequence does not contain any of the illegal restriction sites.

Source

The coding gene ppk1(E.C. 2.7.4.1) is amplified from Tannerella forsythia (ATCC 43037) genomic DNA.

Access Instance Definition
public
public
BBa_K525998
BBa_K1217001
pT7
BBa_K1217001
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1217000/1