Types | DnaRegion
|
Roles | Composite
engineered_region
|
Sequences | BBa_K1217000_sequence (Version 1)
|
Description
Polyphosphate kinase (ppk1) (E.C. 2.7.4.1) catalyzes the formation of polyphosphate from ATP. In BBa_K1217000, ppk1 was under the control of T7 promotor. Under iptg induction, over-expression of ppk1 can reduce the phosphate level in the medium by its incorporation into polyphosphate synthesis inside the bacteria.
Notes
We choose to amplify ppk1 gene from Tannerella forsythia (ATCC 43037) since the sequence does not contain any of the illegal restriction sites.
Source
The coding gene ppk1(E.C. 2.7.4.1) is amplified from Tannerella forsythia (ATCC 43037) genomic DNA.