Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Date created: 2013-09-11 11:00:00
Date modified: 2015-05-08 01:09:44
-- No description --
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1230006_sequence (Version 1) |
Description
The present construction will allow the secretion of an antimicrobial peptide into de medium by the 5??? joining of the PelB leader sequence to the antimicrobial peptide GW-Q5.PelB is a 20 aminoacid N??? terminal sequence which directs the proteins for their exportation into the medium trough type II secretion, in which the protein goes to the periplasm before exportation via the Sec translocation machinery in the outer membrane
GW-Q5 is a cationic alpha-helical peptide designed de novo using the natural peptide Magainin as a template, upon which variants that maintained major structural determinants like electric charge, hydrophobicity and angular momentum.
Notes
PelB and GwQ5 were fused by PCR making a Reverse primer which added to the 3' extreme of PelB the first 20 base pairs of GW-Q5.Source
PelBQ5 was obtained from biopart BBa_J32015.GW-Q5 was obtained de novo by Chou et al. (2008)
H.-T. Chou et al. / International Journal of Antimicrobial Agents 32 (2008) 130???138
| Access | Instance | Definition |
| public public | BBa_J32015 BBa_K1230005 | PelB BBa_K1230005 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J32015 BBa_K1230005 | 1,66 75,137 | PelB BBa_K1230005 |