Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andr??s Ferri??o Iriarte
Date created: 2013-09-25 11:00:00
Date modified: 2015-05-08 01:09:44
araC-pBad-rbs-LL37-rbs-GFP-pLacI-rbs-MarA Generator
| Types | DnaRegion |
| Roles | Project engineered_region |
| Sequences | BBa_K1230015_sequence (Version 1) |
Description
LL-37 antimicrobial peptide with human codon usage under the Arabinose promoter with GFP generator. The MarA protein acts as a transcriptional activator in E. coli. It allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to cationic antimicrobial peptides, such as LL-37. This composite part allows MarA expression in presence of IPTG, by using the LacI promoter and the RBS based on Elowitz repressilator.Notes
LL-37 has the codon usage for humans. Using E. coli codon usage may inhibit protein function by wrong folding.Source
Genscript2013 DNA distribution
| Access | Instance | Definition |
| public public | BBa_K1230011 BBa_K1230014 | BBa_K1230011 BBa_K1230014 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1230011 BBa_K1230014 | 1,1355 1364,2868 | BBa_K1230011 BBa_K1230014 |