Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andr??s Ferri??o Iriarte
Date created: 2013-09-25 11:00:00
Date modified: 2015-05-08 01:09:44
pLacI-rbs-MarA-rbs-GFP-double terminator-pLsrA-rbs-LL-37
| Types | DnaRegion |
| Roles | Project engineered_region |
| Sequences | BBa_K1230016_sequence (Version 1) |
Description
General DesignOur design has two systems:
The Antimicrobial Peptide System, which produces the peptide LL-37; and
The Resistance and Expulsion System which produces the MarA protein, which activates the arcAB & TolC operons, activating the arcAB & TolC efflux pump.
Antimicrobial Peptide System
H. pylori and other pathogenic bacteria produce AI-2, a quorum sensing molecule. This signal is detected by the pLsrA promoter (BBa_K117002), which allows the transcription of LL37 (BBa_K1230001), which is traduced and then translated into the antimicrobial peptide LL37. Once LL37 is expelled by the ArcAB-TolC efflux pump, it can act against H. pylori.
Resistance & Expulsion System
This system is induced by promoter LacI (BBa_R0010), which allows the transcription of marA gene (BBa_K1230000), producing the protein MarA. MarA acts on the arcAB and tolC operons, which activates de ArcAB-TolC efflux pump, allowing the expulsion of LL37 peptide.
Notes
LL-37 has the codon usage for humans. Using E. coli codon usage may inhibit protein function by wrong folding.Source
Genscript2013 DNA distribution.
| Access | Instance | Definition |
| public public | BBa_K1230004 BBa_K1230012 | BBa_K1230004 BBa_K1230012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1230004 BBa_K1230012 | 1,619 628,1741 | BBa_K1230004 BBa_K1230012 |