Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Luning Yang
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:09:47
A reporter for the TALE protein binding ability
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1311007_sequence (Version 1) |
Description
It is a reporter for the TALE(TranscriptionActivator???Like Effectors) protein binding ability. It consists of tandem three TALE binding sequence(ACCTCATCAGGAACATGTT) repeats in the RBS of LacI expressing cassette.Notes
Promoter(J23102)-TALE binding site(repeats three times)-RBS-LacI coding sequence-Terminator(B0015)-LacI Regulative Promoter(R0010)-RBS(B0034)-mRFP1(E1010)-Terminator(B0015)Source
It comes from other parts and a short oligonucleotide sequence as the TALE binding site.| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23102 TALE binding site+RBS BBa_C0012 lacI-LVA BBa_B0015 stem_loop polya stop BBa_J04450 BBa_R0010 CAP -35 -10 LacI start BBa_B0034 conserved BBa_E1010 mrfp1 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop | 1,35 42,128 135,1169 135,1169 1203,1331 1214,1257 1318,1331 1324,1324 1340,2408 1340,1539 1428,1465 1476,1481 1500,1505 1505,1539 1512,1512 1548,1559 1552,1555 1566,2246 1566,2240 2280,2359 2291,2334 2368,2408 2375,2394 2395,2408 2401,2401 | feature/BioBrick engineered_region sequence_feature feature/misc engineered_region feature/BioBrick CDS feature/cds engineered_region feature/BioBrick feature/stem_loop stem_loop polyA_site feature/polya stop_codon feature/stop engineered_region feature/BioBrick feature/BioBrick engineered_region feature/binding non_covalent_binding_site feature/promoter promoter feature/promoter promoter feature/binding non_covalent_binding_site feature/start start_codon feature/BioBrick engineered_region conserved_region feature/conserved engineered_region feature/BioBrick CDS feature/cds feature/BioBrick engineered_region feature/stem_loop stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop polyA_site feature/polya feature/stop stop_codon |