Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yutaka Murata
Date created: 2014-10-03 11:00:00
Date modified: 2015-05-08 01:09:47
pSB1C3 plus 6x His tag
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K1312004_sequence (Version 1) |
Description
This part is pSB1C3 which introduced 6xHis tag and stop codon UAA after Biobrick Suffix. Insert promoter,RBS and coding protein gene which is from start codon to before stop codon, if you want to add 6xHis tag to protein.Notes
We added 6xHistag and stop codon UAA to after Biobrick Suffix.Source
pSB1C3| Sequence Annotation | Location | Component / Role(s) |
| 6xHistag stop codon Biobrick suffix E. coli his operon terminator Stem loop Verification reverse (VR) primer binding site rep (pMB1) ORI T0 terminator Chloramphenicol resistance Verification forward (VF2) primer binding site end of terminator Stem loop BioBrick prefix | 22,39 40,42 1,21 43,114 51,85 155,174 282,896 297,297 1063,1168 1181,1840 1954,1973 2030,2033 2053,2062 2072,2091 | feature/tag tag feature/stop stop_codon sequence_feature feature/dna stem_loop feature/stem_loop stem_loop feature/stem_loop primer_binding_site feature/primer_binding feature/misc sequence_feature feature/misc sequence_feature feature/stem_loop stem_loop CDS feature/cds primer_binding_site feature/primer_binding feature/stem_loop stem_loop stem_loop feature/stem_loop sequence_feature feature/dna |