BBa_K1315010

BBa_K1315010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1315010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dimitrios Michailidis
Date created: 2014-09-25 11:00:00
Date modified: 2015-05-08 01:09:50

Pseudomonas AutoInducer 1 (PAI-1) Biosensor



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1315010_sequence (Version 1)

Description

This is a composite part that consists of 4 biobricks. This is an alternative PA1 Activated System from Bba_K1315009 that instead uses pluxR (Bba_R0062). In the front it has ptet(Bba_R0040) which is constitutively on. This produces LasR all the time with lasR(Bba_C0179) next in the sequence. Only in the presence of the Pseudomonas Autoinducer 1 (PA1), LasR forms an complex with PA1 and it can then activate the pluxR(Bba_R0062) promoter that leads to the production of GFP(Bba_E0040).

Notes

All the inserts were digested with XbaI and PstI, and all the vectors were each time cut with SpeI and PstI.

Source

All the parts have come from existing iGEM distribution kits.

Access Instance Definition
public
public
public
public
BBa_R0040
BBa_C0179
BBa_R0062
BBa_E0040
p(tetR)
lasR
lux pR
GFP
igem#experience Issues  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K1315010/1