| Types | DnaRegion
|
| Roles | engineered_region
Composite
|
| Sequences | BBa_K1315010_sequence (Version 1)
|
Description
This is a composite part that consists of 4 biobricks. This is an alternative PA1 Activated System from Bba_K1315009 that instead uses pluxR (Bba_R0062). In the front it has ptet(Bba_R0040) which is constitutively on. This produces LasR all the time with lasR(Bba_C0179) next in the sequence. Only in the presence of the Pseudomonas Autoinducer 1 (PA1), LasR forms an complex with PA1 and it can then activate the pluxR(Bba_R0062) promoter that leads to the production of GFP(Bba_E0040).
Notes
All the inserts were digested with XbaI and PstI, and all the vectors were each time cut with SpeI and PstI.
Source
All the parts have come from existing iGEM distribution kits.
