Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joan Cortada Garcia
Date created: 2014-09-13 11:00:00
Date modified: 2015-05-08 01:09:50
T7 lacO + mtrCAB
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1316012_sequence (Version 1) |
Description
Combination of T7 promoter with the lac operator. For the promoter to be active both T7 RNA polymerase and the lac operon inducer (lactose or an analogue such as IPTG) must be present. This double regulatory system reduces the promoter leakageMtrCAB is an electron transport complex of the bacteria Shewanella oneidensis
Notes
In order to eliminate the illegal restriction sites, the entire construct was PCRed in different parts and reassembled via the Golden Gate Assembly methodSource
Caroline M. Ajo-Franklin laboratory (Goldbeck et al. ACS Synth. Biol. 2013, 2, 150−159)| Sequence Annotation | Location | Component / Role(s) |
| T7 lacO promoter RBS mtrC start stop mtrA start stop mtrB start stop | 29,76 105,109 129,2141 129,131 2139,2141 2210,3211 2210,2212 3209,3211 3224,5317 3224,3226 5315,5317 | promoter feature/promoter feature/rbs ribosome_entry_site feature/cds CDS start_codon feature/start feature/stop stop_codon CDS feature/cds start_codon feature/start stop_codon feature/stop CDS feature/cds feature/start start_codon feature/stop stop_codon |