Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joan Cortada Garcia
Date created: 2014-09-28 11:00:00
Date modified: 2015-05-08 01:09:50
pRhamnose regulating csgB + J23110 constitutive Anderson promoter regulating csgA
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1316013_sequence (Version 1) |
Description
This part is meant to constitutively express the curli gene csgB which codes for the production of the curli protein that is anchored to the cell membrane (CsgB).The csgA gene codes for the major curli protein (CsgA), which forms long fibrils with other CsgA monomers in a self-assembly process. These CsgA long fibrils bind to the anchored CsgB protein forming the amyloid-like curli structure.
As many more CsgA than CsgB subunits are required for curli formation, this part intends to have an already high amount of CsgA present in the cell (constitutively expressed) and, once the cell is induced (with Rhamnose in this case), only CsgB protein is required to be expressed for curli formation, thus leading to a faster curli generation process.
Notes
Many more CsgA protein subunits are required compared to CsgB in curli formation.Source
csgA and csgB were PCRed out of the K540000 Lyon 2011 iGEM part.| Access | Instance | Definition |
| public public | BBa_K1316018 BBa_K1316019 | BBa_K1316018 BBa_K1316019 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1316018 BBa_K1316019 | 1,535 544,1040 | BBa_K1316018 BBa_K1316019 |