Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joan Cortada Garcia
Date created: 2014-09-28 11:00:00
Date modified: 2015-05-08 01:09:50
T7 lacO + mtrCAB with His tag
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K1316017_sequence (Version 1) |
Description
Combination of T7 promoter with the lac operator. For the promoter to be active both T7 RNA polymerase and the lac operon inducer (lactose or an analogue such as IPTG) must be present. This double regulatory system reduces the promoter leakage MtrCAB is an electron transport complex of the bacteria Shewanella oneidensisA His tag was placed at the C-terminus of the MtrB protein in order to easily be able to detect and purify this protein.
Notes
A His tag was placed at the C-terminus of the MtrB protein in order to easily be able to detect and purify this protein.The big gene complex is supposed to be very toxic for the cell, thus, having a low leakage promoter such as the T7 lacO is crucial to ensure the viability of the cells.
Source
Caroline M. Ajo-Franklin laboratory (Goldbeck et al. ACS Synth. Biol. 2013, 2, 150−159)| Sequence Annotation | Location | Component / Role(s) |
| T7 lacO promoter RBS mtrC stop mtrA stop mtrB HisTag | 27,82 103,108 128,2140 2138,2140 2208,3210 3208,3210 3223,5333 5313,5331 | promoter feature/promoter feature/rbs ribosome_entry_site feature/cds CDS feature/stop stop_codon feature/cds CDS feature/stop stop_codon feature/cds CDS tag feature/tag |