Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mounir Benkoulouche
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:09:50
Fusion protein RFP + Elastin like polypeptide (ELP)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1317004_sequence (Version 1) |
Description
The ELP presents specific properties: it precipitates at high temperature. This property can be used for thermal cycling purification for instance. By successive cycles of temperature, ELP can be purified from a mix of proteins.This special property has been used to create a composite part for the iGEM registry. It is a proof of concept of the use of this part as a tool for protein purification by fusing ELP to another protein.
The protein used as an example is RFP, to ease vizualisation of the process.
Notes
This part has been assembled by chew back assembly (cf wiki page: )In-silico cloning and design have been performed on Genome Compiler.
Source
The source of this part is BBa_K1317003.| Access | Instance | Definition |
| public public | BBa_K1317003 BBa_E1010 | BBa_K1317003 mRFP1 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1317003 BBa_E1010 | 1,333 340,1045 | BBa_K1317003 mRFP1 |