Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Florea
Date created: 2014-10-19 11:00:00
Date modified: 2015-05-08 01:09:52
sfGFP fused to CBDclos driven by LacI in pSEVA331-Bb
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1321296_sequence (Version 1) |
Description
A LacI-promoter expression construct of super-folder GFP fused N-terminally to CBDclos- a cellulose binding domain(part BBa_K1321356) in broad host-range plasmid pSEVA331-bb (part BBa_K1321300) which replicates in the cellulose producing bacterium G.xylinusBBa_K1321356 is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. BBa_K1321356 in pSEVA331-Bb is part of the G.xylinus genetic engineering toolbox.
G.xylinus toolbox was designed to ease genetic engineering of cellulose-based biomaterials using the cellulose synthesizing bacterium Gluconacetobacter xylinus (parts Bba_K1321305 and BBa_K1321306) by providing a collection of widely used parts in pSEVA331-Bb backbone. pSEVA331-Bb is a non-standard broad host range plasmid capable of replication in G.xylinus and E.coli (a shuttle vector) and was selected as the registry's standard plasmid backbone pSB1C3 can not be used for G.xylinus engineering.
NOTE: Because the registry's standard plasmid backbone pSB1C3 is not capable of replication in Gluconacetobacter species, the G.xylinus genetic engineering toolbox is housed mainly in pSEVA331-Bb. pSEVA331-Bb is a non-standard backbone, which therefore can't be quality controlled by and maintained in the registry. However, in order to make the G.xylinus toolbox available for the synthetic biology community, Imperial iGEM 2014 team has made the toolbox freely available upon request, with quality control provided (see Experience). For requests, please contact Imperial iGEM 2014 team.
Notes
BBa_K1321296 was created by restricting BBa_K1321356 and BBa_K1321300 with PstI and XbaI, gel purifying the resulting fragments, ligating using T4 DNA ligase and transforming the ligase products into chemically competent DH10B cells.Source
BBa_K1321356 and BBa_K1321300| Access | Instance | Definition |
| public public | BBa_K1321356 BBa_K1321300 | BBa_K1321356 BBa_K1321300 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1321356 BBa_K1321300 | 1,1252 1261,4153 | BBa_K1321356 BBa_K1321300 |