Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Florea
Date created: 2014-10-19 11:00:00
Date modified: 2015-05-08 01:09:52
J23101-GFP in pSEVA321-Bb
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1321299_sequence (Version 1) |
Description
This RFP expressed behind a lacI regulated promoter (part BBa_R0010) in pSEVA331-Bb plasmid backbone (part BBa_K1321300). This construct is a member of the G.xylinus genetic engineering toolbox.G.xylinus toolbox was designed to ease genetic engineering of cellulose-based biomaterials using the cellulose synthesizing bacterium Gluconacetobacter xylinus (parts Bba_K1321305 and BBa_K1321306) by providing a collection of widely used parts in pSEVA331-Bb backbone. pSEVA331-Bb is a non-standard broad host range plasmid capable of replication in G.xylinus and E.coli (a shuttle vector) and was selected as the registry's standard plasmid backbone pSB1C3 can not be used for G.xylinus engineering.
NOTE: Because the registry's standard plasmid backbone pSB1C3 is not capable of replication in Gluconacetobacter species, the G.xylinus genetic engineering toolbox is housed mainly in pSEVA331-Bb. pSEVA331-Bb is a non-standard backbone, which can't be quality controlled and thus maintained by the registry. However, in order to make the G.xylinus toolbox available for the synthetic biology community, Imperial iGEM 2014 team has made the toolbox freely available upon request, with quality control provided (see Experience). For requests, please contact Michael Florea at mf1512@imperial.ac.uk.
Notes
BBa_K1321330 was created by restricting BBa_K1321300 and BBa_R0010 with PstI and XbaI, gel purifying the resulting fragments, ligating using T4 DNA ligase and transforming the ligation reaction into E.coli DH10B.Source
This is a composite part of BBa_I20260 and BBa_K1321300| Access | Instance | Definition |
| public public | BBa_I20260 BBa_K1321301 | BBa_I20260 BBa_K1321301 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I20260 BBa_K1321301 | 1,919 928,4520 | BBa_I20260 BBa_K1321301 |