Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xenia Spencer-Milnes
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:09:53
Linker-CBDcipA-Linker + sfGFP with LacI promoter
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1321371_sequence (Version 1) |
Description
A LacI-promoter expression construct of super-folder GFP fused C-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our project page for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below:
Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.
Notes
Linker either side of the CBD so fusions should be feasible at both N and C-terminiSource
This is cloned from the specified biobricks| Access | Instance | Definition |
| public public public public public | BBa_J04500 BBa_K1319106 BBa_K1321014 BBa_B0105 BBa_K1321337 | BBa_J04500 Scar25 ATG BBa_K1321014 Scar 25 BBa_K1321337 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_K1319106 BBa_K1321014 BBa_B0105 BBa_K1321337 | 1,220 221,235 236,946 947,952 953,1663 | BBa_J04500 Scar25 ATG BBa_K1321014 Scar 25 BBa_K1321337 |