Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tianyi Li
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:09:54
HXT1-CMVmini-EGFP-tADH1
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1328001_sequence (Version 1) |
Description
This is a composite part containing a glucose-sensitive promoter (BBa_K1328000) and a EGFP reporter with a tADH1 transcription terminator.S. cerevisiae cells transformed with this part was cultured in glucose-free medium with glycerol as carbon-source for 8 hrs. They were then cultured for an additional hour with varying glucose levels (0, 5, 15, 25 mmol/L). A increasing level of GFP expression was observed with increasing levels of glucose concentration, in both microscopy and flow cytometry observations.
Notes
Sequence contains EcoRI and PstI restriction sites, consider using XbaI and SpeI for cloning.Source
Promoter region is assembled from parts: HXT1-CMVmini promoter (BBa_K1328001), EGFP (BBa_K1123017) and tADH1 transcription terminator.| Access | Instance | Definition |
| public public | BBa_K1328000 BBa_K1123017 | BBa_K1328000 BBa_K1123017 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1328000 BBa_K1123017 | 1,790 797,1513 | BBa_K1328000 BBa_K1123017 |