BBa_K1334025

BBa_K1334025 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1334025
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haoyuan Sun
Date created: 2014-09-28 11:00:00
Date modified: 2015-05-08 01:09:58

IPTG inducible E7 protein generator



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1334025_sequence (Version 1)

Description

K1334025 is composed of three main parts. A constitutive lacI repressor generator(J23106,P0412), an inducable promoter which can be activated by adding IPTG(R0011), and an E7 lysis protein which can result in the lysis of E.coli if being expressed. This reconstructed biobrick can be used to prove the function of E7. When IPTG is added to the mixture, the growth rate of E.coli drops. Besides, this part can also be used to help the release of a target protein to the exterior of cell when you add such a protein part(with its independent promoter) to the end of this part.

Notes

None.

Source

All the parts of this reconstructed biobrick come from the iGEM registry.

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1334025/1