BBa_K136015

BBa_K136015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K136015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: alexandra bouaziz

Date created: 2008-10-24 11:00:00

Date modified: 2015-05-08 01:10:03

pBad/araC - GFP tripart

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K136015_sequence (Version 1)

Description

pBad is an E. coli promoter that is tightly controlled by:
*inducer: L-arabinose.
* repressor: AraC apparently acts as the repressor

one study concluded that arabinose can change the conformation of araC and prevent it from successfully binding to and repressing pBad.
pBad is followed by a GFP tripart in our construction .

Notes

AracpBAD was digested by SpeI -PstI (insert) and cloned into the psb2k3 that contain the gfp tripart .

Source

flgA promotor comes from E. coli K12 strain MG1655. gfp tripart comes from the registry.

Components

Access	Instance	Definition
public	BBa_I0500	pBad/araC
public	BBa_B0030	BBa_B0030
public	BBa_E0040	GFP
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I0500	1,1210	pBad/araC
BBa_B0030	1219,1233	BBa_B0030
BBa_E0040	1240,1959	GFP
BBa_B0010	1968,2047	BBa_B0010
BBa_B0012	2056,2096	BBa_B0012

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K136015/1

Member of these Collections

• iGEM Parts Registry