BBa_K136030

BBa_K136030 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K136030

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: alexandra bouaziz

Date created: 2008-10-24 11:00:00

Date modified: 2015-05-08 01:10:03

tetR promoter - GFP Tripart

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K136030_sequence (Version 1)

Description

conditional system:signal tet on /tet off

Promoter PTet is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, aTc.
after this promotor we put a rapporter gene .

Notes

psB1A2 are digested by SpeI PstI (BV),then the promotor(insert) was cloned into the J61002 plasmid containing gFP tripart .

Source

Assembly of different parts from the registry.

Components

Access	Instance	Definition
public	BBa_R0040	p(tetR)
public	BBa_B0030	BBa_B0030
public	BBa_E0040	GFP
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	p(tetR)
BBa_B0030	63,77	BBa_B0030
BBa_E0040	84,803	GFP
BBa_B0010	812,891	BBa_B0010
BBa_B0012	900,940	BBa_B0012

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K136030/1

Member of these Collections

• iGEM Parts Registry