Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jourdan Camille
Date created: 2014-09-24 11:00:00
Date modified: 2015-06-08 08:36:05
Pveg - RBS - Antifungal D4E1 - Double Terminator
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1364009_sequence (Version 1) |
Description
This expression cassette is designed for the expression of an antifugal peptide, D4E1, and for its secretion in Bacillus subtilis. D4E1 has shown antifungal activity by complexing with a streol present in condinia's wall of a variety of fungi.It is composed of a constituve promoter (K143012 Pveg), a RBS (K780002 strong RBS), the open reading frame of D4E1 and a double terminator (B0015).
Notes
The coding sequence of D4E1 is flanked on the N-terminal end with a signal peptide (amyE signal peptide) followed by a pro peptide, cleaved during the secretion process.Source
De Lucca AJ,??Bland JM,??Grimm C,??Jacks TJ,??Cary JW,??Jaynes JM,??Cleveland TE,??Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.Can J Microbiol.??1998 Jun;44(6):514-20.
| Access | Instance | Definition |
| public public | BBa_K823003 BBa_K1364003 | BBa_K823003 D4E1 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K823003 BBa_K1364003 | 1,237 246,567 | BBa_K823003 D4E1 |