Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eduardo Cepeda Ca??edo
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:07
Genetic construct for msbB gen deletion
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1366022_sequence (Version 1) |
Description
This biobrick contains the sequence homologies (50bp) to delete msbB gene (LPS moiety carrier) in E. coli with a kanamycin resistance and a Multiple Cloning Site (for the generation of genomic knock-ins of any desired gene in E. coli). The deletion of msbB gene and the replacement of that sequence with the Kan resistance are performed with lambda-red recombination technology. FLP-FRT sequences are included to remove the antibiotic resistance after the recombination with a specific recombinase.Notes
This is the general structure50bp homology msbB// FRT // KanR Promoter // RBS // KanR // Terminator // FRT // MCS // 50 bp homology msbB
Source
We obtain the sequence from NCBI. The accession number is NC_007635.1| Sequence Annotation | Location | Component / Role(s) |
| msbB homology arm (1) FRT KanR promoter RBS KanR Double terminator msbB homology arm (2) FRT MSC | 1,50 51,85 86,131 132,144 145,940 940,1069 1123,1173 1070,1104 1105,1122 | sequence_feature feature/dna feature/dna sequence_feature promoter feature/promoter feature/rbs ribosome_entry_site CDS feature/cds stop_codon feature/stop sequence_feature feature/dna sequence_feature feature/dna feature/scar |