BBa_K1368009

BBa_K1368009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1368009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lu Wang

Date created: 2014-09-28 11:00:00

Date modified: 2015-05-08 01:10:08

Pluxr+taRNA+T+Pluxr+CrRNA+cre

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1368009_sequence (Version 1)

Description

The main purpose of this part is cre protein expression.Then we add the taRNA and crRNA to reduce the leakage of the luxR promoter.

Notes

Then we add the taRNA and crRNA can reduce the leakage of the luxR promoter.But it is not one hundred percent inhibition.

Source

We got the sequence of crRNA from the iGEM website.Then we synthetic gene by ourselves.

Components

Access	Instance	Definition
public	BBa_R0062	lux pR
public	BBa_I714075	BBa_I714075
public	BBa_R0062	lux pR
public	BBa_J01010	Lock 1
public	BBa_J61047	Cre
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0062	1,55	lux pR
BBa_I714075	64,294	BBa_I714075
BBa_R0062	303,357	lux pR
BBa_J01010	366,405	Lock 1
BBa_J61047	412,1448	Cre
BBa_B0015	1457,1585	BBa_B0015

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1368009/1

Member of these Collections

• iGEM Parts Registry