Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Hou
Date created: 2014-09-03 11:00:00
Date modified: 2015-05-08 01:10:08
AgrA+SH3 peptide
P2 promoter+GFP
| Types | DnaRegion |
| Roles | engineered_region Signalling |
| Sequences | BBa_K1369004_sequence (Version 1) |
Description
This sequence is composed of the AgrA response regulator with an SH3 peptide attached to the N-terminus. Downstream of the AgrA coding sequence is a GFP reporter gene which is regulated by the P2 promoter.Notes
A double terminator sequence separates the AgrA sequence from the P2 promoter sequence.Source
The AgrA response regulator and P2 promoter are endogenous to Staphylococcus aureus, which are involved in the AgrBDCA quorum sensing system.| Sequence Annotation | Location | Component / Role(s) |
| RBS AgrA GS linker SH3 pep stop codon double terminator P2 promoter RBS sfGFP pBAD | 286,317 318,941 942,977 978,1010 1011,1019 1026,1154 1167,1262 1263,1302 1303,2019 1,285 | ribosome_entry_site feature/rbs feature/protein CDS feature/misc sequence_feature sequence_feature feature/misc stop_codon feature/stop sequence_feature feature/misc promoter feature/promoter ribosome_entry_site feature/rbs sequence_feature feature/misc feature/promoter promoter |