Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Victoria Hsiao
Date created: 2008-07-27 11:00:00
Date modified: 2015-05-08 01:10:09
Constitutive folB expression construct
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K137053_sequence (Version 1) |
Description
This is the folate synthesis gene folB, one of five genes involved in the folate synthesis operon. In this construct, folB has been inserted behind the strong promoter j23100, the strong RBS b0034, and in front of the double terminator B0015. The vector is the inducible copy plasmid psB2K3, which can be induced to high copy via IPTG.The purpose of this construct is to test the effects of folB (dihydroneopterin aldolase) overexpression on total folate production in Escherichia coli, with the goal of increasing total folate production.
Notes
We had originally planned on overexpressing the entire folate operon, as well as the individual gene components of the operon. However, we were unable to clone the operon out of the genome successfully.Source
The original folB gene was extracted from the Lactoccocus lactis subspe. IL1403 genome (ordered from ATCC).| Access | Instance | Definition |
| public public public public public | BBa_J23100 BBa_B0034 BBa_K137009 BBa_B0010 BBa_B0012 | BBa_J23100 BBa_B0034 folB BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K137009 BBa_B0010 BBa_B0012 | 1,35 44,55 62,415 424,503 512,552 | BBa_J23100 BBa_B0034 folB BBa_B0010 BBa_B0012 |