Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Victoria Hsiao
Date created: 2008-07-27 11:00:00
Date modified: 2015-05-08 01:10:09
Constitutive pabA expression construct
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K137055_sequence (Version 1) |
Description
PabA is one of three genes (pabA, pabB, pabC) involved in the para-aminobenzoic acid (pABA) synthesis pathway from chorismate. In this construct, pabA (para-aminobenzoate synthetase component II) has been placed behind the strong promoter j23100 and the strong RBS b0034, and in front of the terminator b0015. Currently it is in the inducible copy plasmid psb2k3, which can be induced to high copy via IPTG.Notes
The purpose of this construct is to test the effects of pabA overexpression on the total production of pABA from chorismate. pABA is a necessary structural component in the synthesis of folate and so the hope is that by increasing the flux through the pABA synthesis pathway, we will be able to increase the total production of folate.Source
pabA has been extracted from the genome of L.lactis subspe. IL1403.| Access | Instance | Definition |
| public public public public public | BBa_J23100 BBa_B0034 BBa_K137005 BBa_B0010 BBa_B0012 | BBa_J23100 BBa_B0034 pabA BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K137005 BBa_B0010 BBa_B0012 | 1,35 44,55 62,646 655,734 743,783 | BBa_J23100 BBa_B0034 pabA BBa_B0010 BBa_B0012 |