BBa_K137060

BBa_K137060 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K137060

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Allen Lin

Date created: 2008-07-30 11:00:00

Date modified: 2015-05-08 01:10:09

GFP fimE switch with 450 bp tetR promoter

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K137060_sequence (Version 1)

Description

A promoter facing upstream is flanked by two FimE binding sites. GFP is downstream to this fimE switch. Active FimE will flip the promoter such that GFP is expresssed.

Notes

None.

Source

Previously constructed Biobrick parts.

Components

Access	Instance	Definition
public	BBa_K137010	fimE IRL
public	BBa_K137049	BBa_K137049
public	BBa_K137008	IRR
public	BBa_B0034	BBa_B0034
public	BBa_E0040	GFP
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K137010	1,35	fimE IRL
BBa_K137049	44,493	BBa_K137049
BBa_K137008	502,536	IRR
BBa_B0034	545,556	BBa_B0034
BBa_E0040	563,1282	GFP
BBa_B0010	1291,1370	BBa_B0010
BBa_B0012	1379,1419	BBa_B0012

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K137060/1

Member of these Collections

• //function/recombination/fim
• iGEM Parts Registry