BBa_K137077

BBa_K137077 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K137077

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Doug Tischer

Date created: 2008-08-08 11:00:00

Date modified: 2015-05-08 01:10:09

Constitutively produces pyruvate oxidase.

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K137077_sequence (Version 1)

Description

This part constitutively produced pyruvate oxidase (K137069) at a high level. Preliminary experience shows DH10B cells with this construct grow significantly slower than normal in 5ml LB shaking cultures. This could be either due high protein load or perhaps hydrogen peroxide being produced by pyruvate oxidase. More testing is necessary to see if pyruvate oxidase is functional in vivo.

Notes

None.

Source

J23100, B0034 and B0015 are from the registry. Pyruvate oxidase is from a genomic PCR off of Streptococcus pnumoniae R6.

Components

Access	Instance	Definition
public	BBa_J23100	BBa_J23100
public	BBa_B0034	BBa_B0034
public	BBa_K137069	spxB
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23100	1,35	BBa_J23100
BBa_B0034	44,55	BBa_B0034
BBa_K137069	62,1840	spxB
BBa_B0010	1849,1928	BBa_B0010
BBa_B0012	1937,1977	BBa_B0012

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K137077/1

Member of these Collections

• iGEM Parts Registry