Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Victoria Hsiao
Date created: 2008-08-21 11:00:00
Date modified: 2015-05-08 01:10:11
pabA + pabB complete pABA construct
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K137110_sequence (Version 1) |
Description
This construct contains both genes from the para-aminobenzoic acid synthesis pathway, pabA and pabB. The construct contains the constitutive strong promoter j23100, strong RBS b0034, pabA, b0034, pabB, and then the double terminator b0015.Notes
We wanted to test constitutive overexpression of the para-aminobenzoic acid synthesis genes to see if that would improve total folate overexpression levels in E. coli.Source
Both genes were extracted from the genomic DNA of lactococcus lactis.| Access | Instance | Definition |
| public public public public public public public | BBa_J23100 BBa_B0034 BBa_K137005 BBa_B0034 BBa_K137006 BBa_B0010 BBa_B0012 | BBa_J23100 BBa_B0034 pabA BBa_B0034 BBa_K137006 BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K137005 BBa_B0034 BBa_K137006 BBa_B0010 BBa_B0012 | 1,35 44,55 62,646 655,666 673,2562 2571,2650 2659,2699 | BBa_J23100 BBa_B0034 pabA BBa_B0034 BBa_K137006 BBa_B0010 BBa_B0012 |