BBa_K1377001

BBa_K1377001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1377001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jade Minseob Yoo

Date created: 2014-10-08 11:00:00

Date modified: 2015-05-08 01:10:12

spaA backward + sortase A + spaB + spaC

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K1377001_sequence (Version 1)

Description

Pilin A gene cluster is coding for pili A of Corynebacterium diphtheriae. It has four coding regions and one regulatory region. Regulatory region is its own promoter. Four coding regions are sortase A, enzyme that makes pili A and pilin spa A & B & C that are components of pili A . WIth these all regions, we can make pili A.
And we are going to modifying this part to generate "Our Favorite Protein Whip", especially spa A

Notes

Just include all cluster that related to make pili A
And there is no good restriction enzyme unique cut site, so we slice the cluster to two

Source

Genomic DNA of Corynebacterium diphtheriae

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1377001/1

Member of these Collections

• iGEM Parts Registry