Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Stikane
Date created: 2014-09-18 11:00:00
Date modified: 2015-05-08 01:10:16
Plac-RFP(DAS)
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1399013_sequence (Version 1) |
Description
Lactose/IPTG inducible promoter with RFP reporter tagged with DAS-ssrA degradation tag followed by terminator.The tagged RFP is degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
Notes
Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]Source
other biobricks| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 end of LacI coding region (inactive) CAP binding site -35 -10 LacI binding site start conserved BBa_B0034 start RFP DAS-ssrA tag stop stop BBa_K1399003 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_B0015 barcode from BBa_E1010 | 1,200 1,88 89,126 137,142 161,166 166,200 173,173 213,216 209,220 227,229 230,901 902,934 935,937 938,940 227,940 974,1053 985,1028 1062,1102 1069,1088 1089,1102 1095,1095 974,1102 941,965 | engineered_region feature/BioBrick CDS feature/cds feature/binding non_covalent_binding_site promoter feature/promoter feature/promoter promoter non_covalent_binding_site feature/binding start_codon feature/start conserved_region feature/conserved engineered_region feature/BioBrick start_codon feature/start feature/cds CDS tag feature/tag feature/stop stop_codon stop_codon feature/stop feature/BioBrick engineered_region feature/BioBrick engineered_region feature/stem_loop stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop polyA_site feature/polya stop_codon feature/stop engineered_region feature/BioBrick feature/misc sequence_feature |