Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kathleen Lewis
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:10:16
Transcriptional Unit with pTet-pBad tandem promoter
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1401009_sequence (Version 1) |
Description
This transcriptional unit contains the following: pBad-pTet - BCD2 - RFP - B0015. The transcriptional unit is induced by arabinose and atc. This is a Level 1 MoClo part with flanking sites A on the 5' side and site E on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This transcriptional unit has Kanamycin resistance.Notes
This part was created using the MoClo assembly method. The tandem promoters were created by combining two promoters together without any additional sequence in between.Source
Tandem Promoter Reference Paper| Access | Instance | Definition |
| public public public public public | BBa_R0040 BBa_I13453 BBa_K1114107 BBa_E1010 BBa_B0015 | p(tetR) BBa_I13453 BBa_K1114107 mRFP1 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 BBa_I13453 BBa_K1114107 BBa_E1010 BBa_B0015 | 1,54 63,192 201,292 299,1004 1013,1141 | p(tetR) BBa_I13453 BBa_K1114107 mRFP1 BBa_B0015 |