Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ye Chen
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:18
LasR+LasR/PAI1 Inducible promoter+GFP+RhlR(LVA-)+RhlR/PAI2 Inducible promoter+RFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1407016_sequence (Version 1) |
Description
It's composed of seven sections:constitutive production of LasR,LasR/PAI1 Inducible promoter with RBS(B0034)and GFP,terminator,constitutive promoter,RhlR protein generator(LVA-) ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.When PAI1 exsits,the part could be inducibled and expresses GFP protein;When PAI2 exsits,the part could be inducibled and expresses RFP protein.Notes
None.Source
Existing parts on the registry.| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_K553003 BBa_B0034 conserved BBa_C0179 BBa_B0015 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_R0079 BBa_K575009 OP2 OP1 -35 -10 BBa_B0034 conserved GFP protein BBa_E0040 BBa_B0010 BBa_B0015 stem_loop BBa_B0012 T7 TE polya stop BBa_K1407008 BBa_J23119 BBa_B0034 BBa_I1466 conserved BBa_C0171 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop RhlR BBa_R0071 BBa_K575014 -35 -10 start RBS-1\Strong BBa_B0030 RBS mrfp1 BBa_E1010 Help:Barcodes BBa_B0010 BBa_B0015 stem_loop BBa_B0012 T7 TE polya stop BBa_K1407012 | 1,35 1,921 44,55 48,51 62,784 793,921 793,872 804,847 881,921 888,907 908,921 914,914 930,1086 930,1832 975,992 1035,1054 1046,1051 1069,1074 1095,1106 1099,1102 1113,1832 1113,1832 1841,1920 1841,1969 1852,1895 1929,1969 1936,1955 1956,1969 1962,1962 1,1969 1978,2012 2021,2032 2021,2904 2025,2028 2039,2767 2776,2855 2787,2830 2864,2904 2871,2890 2891,2904 2897,2897 2913,2932 2913,2965 2913,3700 2931,2936 2954,2959 2965,2965 2974,2988 2974,2988 2981,2985 2995,3669 2995,3700 3676,3700 3709,3788 3709,3837 3720,3763 3797,3837 3804,3823 3824,3837 3830,3830 1978,3837 | engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick feature/conserved conserved_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region stem_loop feature/stem_loop engineered_region feature/BioBrick feature/stem_loop stem_loop feature/polya polyA_site feature/stop stop_codon feature/BioBrick engineered_region feature/BioBrick engineered_region feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding feature/promoter promoter promoter feature/promoter engineered_region feature/BioBrick conserved_region feature/conserved feature/protein CDS feature/BioBrick engineered_region feature/BioBrick engineered_region engineered_region feature/BioBrick stem_loop feature/stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop polyA_site feature/polya stop_codon feature/stop feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region conserved_region feature/conserved feature/BioBrick engineered_region feature/BioBrick engineered_region feature/stem_loop stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop feature/polya polyA_site feature/stop stop_codon feature/binding non_covalent_binding_site engineered_region feature/BioBrick feature/BioBrick engineered_region feature/promoter promoter promoter feature/promoter start_codon feature/start feature/rbs ribosome_entry_site feature/BioBrick engineered_region feature/rbs ribosome_entry_site feature/cds CDS feature/BioBrick engineered_region feature/barcode engineered_tag feature/BioBrick engineered_region feature/BioBrick engineered_region feature/stem_loop stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop feature/polya polyA_site stop_codon feature/stop engineered_region feature/BioBrick |