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BBa_K1413023

BBa_K1413023 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1413023

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Laura MATABISHI, Nandjafot MENDY

Date created: 2014-10-16 11:00:00

Date modified: 2015-05-08 01:10:18

Promoter-RBS-bphr2 mutated-Terminator

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Details

Types	DnaRegion
Roles	Composite engineered_region
Sequences	BBa_K1413023_sequence (Version 1)

Description

bphR2 gene, from Pseudomonas pseudoalcaligens KF707, allows the transcription of bphR1 which activates the genes of degradation of biphenyls.
bphR2 gene has been mutated because of its pstI site in its sequence.

Notes

Between each part, there is the scar SpeI/xbaI. Moreover, bphr2 gene has been mutated.

Source

J23114, B0034 and terminator come from the 2014 kit distribution
bphr2 has been synthetized (it is the part BBa_K1413021)

Components

Access	Instance	Definition
public public public public public public public	BBa_J23114 BBa_G0001 BBa_B0034 BBa_G0001 BBa_K1413021 BBa_G0001 BBa_B0015	BBa_J23114 scar BBa_B0034 scar BBa_K1413021 scar BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23114 BBa_G0001 BBa_B0034 BBa_G0001 BBa_K1413021 BBa_G0001 BBa_B0015	1,35 44,51 60,71 80,87 94,996 1005,1012 1021,1149	BBa_J23114 scar BBa_B0034 scar BBa_K1413021 scar BBa_B0015

Other Properties

igem#experience	None
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K1413023/1