Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jordan Monk
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:20
The tRNA synthetase/tRNA needed for incorporating o-(2-nitrobenzyl)-L-tyrosine (ONBY) at a UAG codon
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1416000_sequence (Version 1) |
Description
The part includes the gene coding for the Methanocaldococcus jannaschii synthetase mutated to charge the orthogonal tRNA (also in the part) with the non-canonical amino acid o-nitrobenzyl tyrosine (ONBY) along with the proper promoters and terminator . This part encodes for the incorporation of ONBY during translation at AUG amber stop codons in a cell with Release Factor 1 (RF-1) knocked out such as "Amberless" E. coli or "RF-0".Source
Evolved from the Methanocaldococcus janaschii tyrosyl synthetase and tRNA by the Schulz group. Specific mutations sourced from Deiters, A., Groff, D., Ryu, Y., Xie, J. and Schultz, P. G. (2006), A Genetically Encoded Photocaged Tyrosine. Angew. Chem. Int. Ed., 45: 2728???2731.| Sequence Annotation | Location | Component / Role(s) |
| ONBYRS promoter ONBYRS tRNA promoter tRNA terminator | 72,219 233,1153 1330,1379 1396,1471 1497,1526 | promoter feature/promoter CDS feature/protein feature/promoter promoter feature/cds CDS stem_loop feature/stem_loop |