PVeg-gsiB

BBa_K143052 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K143052

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chris Hirst

Date created: 2008-10-05 11:00:00

Date modified: 2015-05-08 01:10:24

Promoter Pveg and RBS gsiB for B. subtilis

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K143052_sequence (Version 1)

Description

Constitutive promoter veg(BBa_K143012) coupled to the strong Ribosome Binding Site gsiB(BBa_K143020) from B. subtilis.

Pveg-gsiB can be used in the context of a Polymerases per second (PoPS) output generator

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Notes

The sequence of Pveg was obtained from the DBTBS1 and RBS-gsiB were obtained from papers2 and the sequence synthesised by GeneArt

Source

Pveg-gsiB was synthesised by GeneArt

Components

Access	Instance	Definition
public	BBa_K143012	Pveg
public	BBa_K143020	RBS-GsiB

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K143012	1,97	Pveg
BBa_K143020	106,116	RBS-GsiB

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K143052/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• iGEM Parts Registry