Pxyl-gsiB

BBa_K143056 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K143056

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chris Hirst

Date created: 2008-10-07 11:00:00

Date modified: 2015-05-08 01:10:24

Promoter xyl and RBS gsiB for B. subtilis

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K143056_sequence (Version 1)

Description

Inducible promoter xyl(BBa_K143014) coupled to the strong Ribosome Binding Site gsiB(BBa_K143020) from B. subtilis.

Pxyl-gsiB can be used to take an input of xylose and give a Ribosomes per second (RiPS) output generator.

Xylose does not directly induce the expression of the promoter xyl, but requires the transcriptional regulator XylR, (BBa_K143036). This means that XylR must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter. XylR is naturally expressed by B. subtilis but should be upregulated to increase efficiency.

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Notes

The sequence of promoter xyl and RBS-gsiB were obtained from papers1 2 3 and the sequence synthesised by GeneArt

Source

Pxyl-gsiB was synthesised by GeneArt

Components

Access	Instance	Definition
public	BBa_K143014	Pxyl
public	BBa_K143020	RBS-GsiB

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K143014	1,82	Pxyl
BBa_K143020	91,101	RBS-GsiB

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K143056/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• iGEM Parts Registry