LacI-T

BBa_K143062 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K143062
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris Hirst
Date created: 2008-09-30 11:00:00
Date modified: 2015-05-08 01:10:24

LacI repressor protein - Terminator



    • Details

    Types
    DnaRegion

    Roles
    Composite

    engineered_region

    Sequences BBa_K143062_sequence (Version 1)

    Description

    LacI transcriptional repressor protein (BBa_K143033) coupled to the double terminator (BBa_B0015.

    The LacI does not possess a LVA degradation tag and gas a short (3 amino acid) N-terminal deletion consistent with LacI used in conjunction with B. subtilis.

    LacI can be used in conjunction with the lac operon promoter (BBa_K143015), where the LacI will act as a receiver for an IPTG input to result in a Polymerases per second (PoPS) output.

    The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.

    The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.

    Notes

    LacI was identified from the pDR111 B. subtilis integration vector. The double terminator is the most commonly used registry terminator.

    Source

    LacI was produced by PCR cloning using Pfu form the B. subtilis integration vector and cloned into a BioBrick with the registry double terminator.

    Access Instance Definition
    public
    public
    public
    		BBa_K143033
    BBa_B0010
    BBa_B0012
    		LacI
    BBa_B0010
    BBa_B0012

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    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K143062/1
     

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