Aad9-T

BBa_K143065 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K143065

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chris Hirst and Imperial iGEM 08

Date created: 2008-10-08 11:00:00

Date modified: 2015-05-08 01:10:24

Spectinomycin Resistance Protein (Aad9) - Terminator

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K143065_sequence (Version 1)

Description

Aad9 spectinomycin resistance protein(BBa_K143031) coupled to the double terminator (BBa_B0015).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Notes

Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source

Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

Components

Access	Instance	Definition
public	BBa_K143031	Aad9
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K143031	1,771	Aad9
BBa_B0010	780,859	BBa_B0010
BBa_B0012	868,908	BBa_B0012

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K143065/1

Member of these Collections

• iGEM Parts Registry