Aad9-T

BBa_K143065 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K143065
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris Hirst and Imperial iGEM 08
Date created: 2008-10-08 11:00:00
Date modified: 2015-05-08 01:10:24

Spectinomycin Resistance Protein (Aad9) - Terminator



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K143065_sequence (Version 1)

Description

Aad9 spectinomycin resistance protein(BBa_K143031) coupled to the double terminator (BBa_B0015).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Notes

Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source

Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

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BBa_K143065/1