Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Chan
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:25
N-terminal GFP w/ an N-terminal Intein
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1435002_sequence (Version 1) |
Description
This part is a N-terminal GFP with an intein fused to it. In the presence of the C-terminal intein conjugated to the C-terminal GFP, the intein will auto catalytically splice itself out and ligate its exteins together, forming the functional GFP.Notes
N/ASource
This part was created using de novo DNA synthesis. The intein sequence was obtained via InBase, a peer-reviewed database of inteins. The intein sequence was isolated from Cyanobacterium synechocystis.| Sequence Annotation | Location | Component / Role(s) |
| His6 Tag Linker N-terminal GFP N-terminal Intein Yeast Terminator E.coli Double Terminator | 1,18 19,30 31,549 550,850 851,1358 1359,1484 | CDS feature/protein feature/protein CDS feature/protein CDS CDS feature/protein stop_codon feature/stop feature/stop stop_codon |