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BBa_K1444015

BBa_K1444015 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1444015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dan Ziemianowicz

Date created: 2014-10-07 11:00:00

Date modified: 2015-05-08 01:10:27

Composite promoter and consensus B. subtilis RBS - pLacI

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Details

Types	DnaRegion
Roles	promoter Regulatory
Sequences	BBa_K1444015_sequence (Version 1)

Description

A composite part consisting of the constitutive promoter Pveg (BBa_K823003), followed by the repressible promoter pLacI (BBa_R0010), and ending with the consensus RBS from B. subtilis (BBa_K090505).

This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pTetR repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed.

Notes

No interference with endogenous cell signalling.

Source

B. subtilis

Components

Access	Instance	Definition
public public public	BBa_K823003 BBa_R0010 BBa_K090505	BBa_K823003 LacI BBa_K090505

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K823003 BBa_R0010 BBa_K090505	1,237 246,445 454,464	BBa_K823003 LacI BBa_K090505

Other Properties

igem#experience	None
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K1444015/1