Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonas Demeulemeester
Date created: 2008-08-19 11:00:00
Date modified: 2015-05-08 01:10:29
FILTER T7 RNA pol
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K145216_sequence (Version 1) |
Description
FILTER This device was designed to filter out noise in an input signal; in our case OmpF PoPs. If there is a short increase in transcription from this promoter, a small amount of J23066 RiboKey is produced. This is then used to unlock (J23078) the short lived UmuD-tagged T7 RNA polymerase. This results in an increase of active T7 protein.The final part of this filter is an AND gate which is used in the components further on in the system. This AND gate consists of a T7 RNA polymerase promoter with another RiboLock (J23078 again). The AND gate will only function if the duration of the noise is sufficiently long and intense (= a signal) in order to have both the short-lived RiboKey AND T7 polymerase available at the same time and thus to produce the component.
Notes
We did a lot of modeling on this device.The most efficient Berkeley 2006 key and lock have been used.
The RiboKy might be required in higher copy number although modeling doesn't predict this.
Source
other parts + synthetic| Access | Instance | Definition |
| public public public public public | BBa_J23109 BBa_J23078 BBa_K145014 BBa_B0010 BBa_B0012 | BBa_J23109 BBa_J23078 T7 BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23109 BBa_J23078 BBa_K145014 BBa_B0010 BBa_B0012 | 1,35 44,95 102,2843 2852,2931 2940,2980 | BBa_J23109 BBa_J23078 T7 BBa_B0010 BBa_B0012 |