Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grzegorz Ścibisz
Date created: 2014-09-30 11:00:00
Date modified: 2015-05-08 01:10:32
PmrA-PmrB(LBT) with terminator (BBa_B1006)
| Types | DnaRegion |
| Roles | engineered_region Intermediate |
| Sequences | BBa_K1459004_sequence (Version 1) |
Description
PmrA-PmrB two-component system is native to Salmonella enterica and in it's native state it is responsible for chemotaxis. PmrB is a transmembrane protein with iron binding peptide on it's extracellular loop. When PmrB binds iron (III) iron, it's intracellular domain gains kinase activity and phosphorylates PmrA, which subsequently binds to pmrC promoter and induces expression of chemotaxis CheZ protein.In this part iron binding tag on the extracellular loop was exchanged with a lanthanide binding tag (LBT), to allow PmrA-PmrB two-component system to respond to lanthanide ions.
Notes
Not many, apart from ligating these two parts together.Source
PmrA-PmrB operon sequence was obtained thanks to kindness of prof. He from the University of Chicago.BBa_B1006 was obtained from 2014 Spring Distribution.
| Access | Instance | Definition |
| public public | BBa_K1459003 BBa_B1006 | BBa_K1459003 BBa_B1006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1459003 BBa_B1006 | 1,1749 1758,1796 | BBa_K1459003 BBa_B1006 |