BBa_K1476000

BBa_K1476000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1476000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Matthew Smith

Date created: 2014-10-08 11:00:00

Date modified: 2015-05-08 01:10:40

PilT1 knockout for Synechocystis sp. PCC 6803

Details

• Types: DnaRegion
• Roles: Other; sequence_feature
• Sequences: BBa_K1476000_sequence (Version 1)

Description

This part deletes the pilT1 ATPase gene in Synechocystis sp. PCC 6803. PilT1 normally allows for pilus retraction.

Notes

The part is designed to contained regions homologous to the pilT1 gene. As such we had to ensure that the homologous region was of sufficient length to allow for recombination. The part contains two regions homologous to pilT1: the first region is homologous to the first 200 bases of pilT1, the second region to the last 200 bases of the pilT1 gene. The centre of the part is a kanamycin resistance gene, which becomes inserted in the middle of the chromosomal pilT1 gene during recombination.

Source

Organism: Cyanobacteria
Genus and Strain: Synechocystis sp. PCC 6803
Source: http://genome.microbedb.jp/cyanobase/Synechocystis/genes/slr0161
Important genotype features: none

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pilT1 left	1,249	sequence_feature, feature/misc
pilT1 right	1126,1374	sequence_feature, feature/misc
KanR	280,1095	sequence_feature, feature/misc

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1476000/1

Member of these Collections

• iGEM Parts Registry