Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Roberto Alejandro Valderrama Alfaro
Date created: 2014-10-07 11:00:00
Date modified: 2015-05-08 01:10:40
Promoter + RBS + TALEN + Terminator
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1480000_sequence (Version 1) |
Description
This is a synthesized construction that encodes for a Transcription Activator-Like Effector Nuclease (TALEN). The gene is under Bba_J23110 constitutive promoter. TALEN enzyme is useful when you want to make a double strand break in an specific site of the DNA. It can be used with the help of primers to add the target site into your vector and then make a loss of function when adding the TALEN due to the double strand break. It also can be used on eukaryotic models to make genome editing. The target nucleotide sequence of the TALEN is GGAGCCATCTGGCCGGGTNotes
Due the target sequence is based on a eukaryotic sequence, TALEN can bind to an undesirable site.Source
Synthetic part based on an eukaryotic gene.| Access | Instance | Definition |
| public public public public | BBa_J23110 BBa_B0030 BBa_K1480001 BBa_B0015 | BBa_J23110 BBa_B0030 BBa_K1480001 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23110 BBa_B0030 BBa_K1480001 BBa_B0015 | 1,35 44,58 65,3091 3100,3228 | BBa_J23110 BBa_B0030 BBa_K1480001 BBa_B0015 |