Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danielle Peters
Date created: 2014-09-25 11:00:00
Date modified: 2015-05-08 01:10:44
Constitutive Dead ER Intein T7 RNAP with YFPH
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1491036_sequence (Version 1) |
Description
T7 RNA polymerase that contains the dead version estrogen receptor intein with a constitutive promoter. YFP is added to the end of the plasmid so that the amount of T7 RNA polymerase that is produced can be quantified through fluorescence analysis of YFP. Used as a control.Notes
Constitutive dead ER Intein T7 RNAP with YFP for quantification of T7 RNAP to use as control for WT InteinSource
The promoter is BBa_J23115, the RBS is BBa_B0034, the T7 RNA polymerase is BBa_K145001, the dead ER Intein is BBa_K1491026, the YFP is BBa_K864100| Access | Instance | Definition |
| public public public public | BBa_J23115 BBa_B0034 BBa_K1491027 BBa_K1491005 | BBa_J23115 BBa_B0034 BBa_K1491027 BBa_K1491005 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23115 BBa_B0034 BBa_K1491027 BBa_K1491005 | 1,35 44,55 62,4319 4328,5086 | BBa_J23115 BBa_B0034 BBa_K1491027 BBa_K1491005 |