BBa_K1491036

BBa_K1491036 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1491036

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Danielle Peters

Date created: 2014-09-25 11:00:00

Date modified: 2015-05-08 01:10:44

Constitutive Dead ER Intein T7 RNAP with YFPH

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K1491036_sequence (Version 1)

Description

T7 RNA polymerase that contains the dead version estrogen receptor intein with a constitutive promoter. YFP is added to the end of the plasmid so that the amount of T7 RNA polymerase that is produced can be quantified through fluorescence analysis of YFP. Used as a control.

Notes

Constitutive dead ER Intein T7 RNAP with YFP for quantification of T7 RNAP to use as control for WT Intein

Source

The promoter is BBa_J23115, the RBS is BBa_B0034, the T7 RNA polymerase is BBa_K145001, the dead ER Intein is BBa_K1491026, the YFP is BBa_K864100

Components

Access	Instance	Definition
public	BBa_J23115	BBa_J23115
public	BBa_B0034	BBa_B0034
public	BBa_K1491027	BBa_K1491027
public	BBa_K1491005	BBa_K1491005

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23115	1,35	BBa_J23115
BBa_B0034	44,55	BBa_B0034
BBa_K1491027	62,4319	BBa_K1491027
BBa_K1491005	4328,5086	BBa_K1491005

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1491036/1

Member of these Collections

• iGEM Parts Registry