BBa_K1497003

BBa_K1497003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1497003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen

Date created: 2014-10-10 11:00:00

Date modified: 2015-05-08 01:10:45

B0034-CHI - Chalcone Isomerase from Petunia with strong RBS

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K1497003_sequence (Version 1)

Description

The Chalcone Isomerse catalyzes the reaction from Naringenin chalcone to Naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin.

For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.

Notes

no

Source

Petunia

Components

Access	Instance	Definition
public	BBa_B0034	BBa_B0034
public	BBa_K1497000	BBa_K1497000

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034	1,12	BBa_B0034
BBa_K1497000	19,744	BBa_K1497000

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1497003/1

Member of these Collections

• //cds/biosynthesis
• //cds/biosynthesis/anthocyanins
• iGEM Parts Registry