BBa_K1500004

BBa_K1500004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1500004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kevin Yang

Date created: 2014-10-16 11:00:00

Date modified: 2015-05-08 01:10:46

IPTG-GFP MutD

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K1500004_sequence (Version 1)

Description

The MutD gene cloned into the IPTG-GFP plasmid for characterization. It is unmutated and still requires the MutD51 (L73W) mutation to become functional in its mutagenic dominant negative form. MutD (dnaQ) gene product controls the editing capacity of pol III (a proofreading DNA exonuclease) and mutD5 mutation leads to a reduction in mismatch repair.

Notes

None

Source

PCR'd from DH5-alpha genomic sequence, with RBS.3 (medium) added as an overhang in front of the gene.

Components

Access	Instance	Definition
public	BBa_K1500002	BBa_K1500002
public	BBa_B0032	BBa_B0032
public	BBa_K1500998	BBa_K1500998

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1500002	1,946	BBa_K1500002
BBa_B0032	955,967	BBa_B0032
BBa_K1500998	974,1705	BBa_K1500998

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1500004/1

Member of these Collections

• iGEM Parts Registry